N myristoylation of the spleen necrosis virus matrix protein is required for correct association of the Gag polyprotein with intracellular membranes and for particle formation.

Abstract excerpt

To determine whether myristoylation is required for spleen necrosis virus replication, we constructed a substitution mutation in the gag gene that alters the putative myristate acceptor glycine residue. This single amino acid change was lethal for virus replication, resulted in aberrant proteolytic processing, and interrupted virion assembly and the release of virus from cells. Immunofluorescence analysis...

Topics

• Animals
• Cell Line
• Codon
• DNA, Viral
• Gene Products, gag
• Genes, gag
• Intracellular Membranes
• Mutation
• Myristic Acid
• Myristic Acids
• Protein Binding