Ras binding to a C-terminal region of GAP.

doi:10.1016/0014-5793(95)00657-u

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Article

Ras binding to a C-terminal region of GAP.

FEBS letters - 17 Jul 1995

Molloy D P, Owen D, Grand R J

Abstract excerpt

Using fluorescence spectroscopy we have identified a binding region for Ras on the GTPase activating protein (GAP) lying within residues 715-753. A synthetic peptide Y922, corresponding to residues 716-753 of GAP binds to wild type Ras showing 3.3-fold higher affinity for the GTP- over the GDP-bo...

Topics

Amino Acid Sequence
Fluorescent Dyes
GTP Phosphohydrolases
GTPase-Activating Proteins
Guanosine Diphosphate
Guanosine Triphosphate
Humans
Kinetics
Magnesium
Molecular Sequence Data
Mutation
Naphthalenesulfonates
Oncogene Protein p21(ras)
Peptide Fragments
Proteins
Recombinant Fusion Proteins
ras GTPase-Activating Proteins

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