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Article

Unravelling the mechanism of dual-specificity GAPs.

The EMBO journal - 7 Apr 2010

Sot Begoña, Kötting Carsten, Deaconescu Delia, Suveyzdis Yan, Gerwert Klaus, Wittinghofer Alfred

Abstract excerpt

The molecular mechanism by which dual-specificity RasGAPs of the Gap1 subfamily activate the GTP hydrolysis of both Rap and Ras is an unresolved phenomenon. RasGAPs and RapGAPs use different strategies to stimulate the GTPase reaction of their cognate G-proteins. RasGAPs contribute an arginine finger to orient through the Gln61 of Ras the nucleophilic water molecule. RapGAP contributes an asparagine (Asn thumb)...

Topics

Amino Acid Sequence
GTPase-Activating Proteins
Guanosine Triphosphate
Humans
Models, Molecular
Molecular Sequence Data
Mutation
Protein Binding
Protein Structure, Tertiary
Receptors, Cytoplasmic and Nuclear
Spectroscopy, Fourier Transform Infrared

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

rap GTP-Binding Proteins

ras GTPase-Activating Proteins

ras Proteins

Source

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Identifiers

PMID: 20186121
DOI: 10.1038/emboj.2010.20
PMCID: PMC2857463

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