Article
Biochemical characterization of yeast RAS2 mutants reveals a new region of ras protein involved in the interaction with GTPase-activating proteins.
The Journal of biological chemistry - 18 Feb 1994
Wood D R, Poullet P, Wilson B A, Khalil M, Tanaka K, Cannon J F, Tamanoi F
Abstract excerpt
We report biochemical characterization of two recently identified mutants of yeast RAS2, RAS2-E99K and RAS2-E130K. These mutants exhibit dominant activating phenotypes in yeast. Characterization of their intrinsic GTPase and GDP dissociation as well as their ability to stimulate adenylate cyclase...
Topics
- Adenylyl Cyclases
- Amino Acid Sequence
- Fungal Proteins
- GTP Phosphohydrolases
- GTPase-Activating Proteins
- Guanosine Diphosphate
- Hot Temperature
- Kinetics
- Models, Molecular
- Mutation
- Phenotype
- Protein Binding
- Protein Conformation
- Proteins
- Saccharomyces cerevisiae