Article
Structural perturbation of the transmembrane region interferes with calcium binding by the Ca2+ transport ATPase.
The Journal of biological chemistry - 5 Jul 1991
Sumbilla C, Cantilina T, Collins J H, Malak H, Lakowicz J R, Inesi G
Abstract excerpt
The Ca(2+)-ATPase of sarcoplasmic reticulum reacts with N-cyclohexyl-N'-(4-dimethylamino-1-naphthyl) carbodiimide (NCD4) yielding a fluorescence labeling that interferes with calcium binding to activating and transport sites of the enzyme and, thereby, with Ca(2+)-dependent ATPase activity. On th...
Topics
- Amino Acid Sequence
- Biological Transport
- Calcium
- Calcium-Transporting ATPases
- Carbodiimides
- Cell Membrane
- Electrophoresis, Polyacrylamide Gel
- Fluorescence Polarization
- Hydrolysis
- Molecular Sequence Data
- Mutation