Article
Assessment of collagen type II induced arthritis in mice by whole blood chemiluminescence.
Autoimmunity - 1 Jan 1994
Miesel R, Dietrich A, Ulbrich N, Kroeger H, Mitchison N A
Abstract excerpt
Lucigenin-enhanced chemiluminescence can be measured in 100 microliters samples of whole, unseparated mouse blood. A procedure for doing so is here described in detail, using a standard clinical luminometer. The assay measures the TPA-induced oxidative burst from granulocytes and macrophages, which is believed to depend on the overall level of inflammation in the body. It is here applied to mice suffering from...
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