Article
A genetic screen identifies cellular factors involved in retroviral -1 frameshifting.
Proceedings of the National Academy of Sciences of the United States of America - 3 Jul 1995
Lee S I, Umen J G, Varmus H E
Abstract excerpt
To identify cellular factors that function in -1 ribosomal frameshifting, we have developed assays in the yeast Saccharomyces cerevisiae to screen for host mutants in which frameshifting is specifically affected. Expression vectors have been constructed in which the mouse mammary tumor virus gag-pro frameshift region is placed upstream of the lacZ gene or the CUP1 gene so that the reporters are in the -1 frame...
Topics
- Adaptor Proteins, Signal Transducing
- Amino Acid Sequence
- Base Sequence
- Blotting, Western
- Carrier Proteins
- Cloning, Molecular
- Frameshift Mutation
- Fungal Proteins
- Genes, Fungal
- Genes, Viral
- Genes, gag