Article
Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant.
Development (Cambridge, England) - 1 Apr 1994
Levine E, Lee C H, Kintner C, Gumbiner B M
Abstract excerpt
E-cadherin function was disrupted in vivo in developing Xenopus laevis embryos through the expression of a mutant E-cadherin protein lacking its cytoplasmic tail. This truncated form of E-cadherin was designed to act as a dominant negative mutant by competing with the extracellular interactions of wild-type endogenous E-cadherin. Expression of truncated E-cadherin in the early embryo causes lesions to develop in...
Topics
- Amino Acid Sequence
- Animals
- Cadherins
- Cell Adhesion
- Gastrula
- Gene Expression
- Genes, Dominant
- Immunoblotting
- Molecular Sequence Data
- Morphogenesis
- Mutation
- Xenopus