Article
Location of N2,N2-dimethylguanosine-specific tRNA methyltransferase.
Biochimie - 1 Jan 1995
Rose A M, Belford H G, Shen W C, Greer C L, Hopper A K, Martin N C
Abstract excerpt
Most steps in the maturation of nuclear coded tRNAs occur in the nucleus in eukaryotic cells, but little is known as to the intranuclear location of this RNA maturation pathway. Indirect immunofluorescence experiments using antibody to N2,N2 dimethylguanosine-specific tRNA methyltransferase, a tRNA processing enzyme, and to Nup1p, a nuclear pore protein, show that both locate to the nuclear periphery in wild type...
Topics
- Amino Acid Sequence
- Blotting, Western
- Cell Fractionation
- Cell Nucleus
- Cytosol
- Deoxyribonucleases
- Endoribonucleases
- Fluorescent Antibody Technique
- Membrane Proteins
- Mitochondria
- Molecular Sequence Data