Article
Nuclear transport defects and nuclear envelope alterations are associated with mutation of the Saccharomyces cerevisiae NPL4 gene.
Molecular biology of the cell - 1 Nov 1996
DeHoratius C, Silver P A
Abstract excerpt
To identify components involved in nuclear protein import, we used a genetic selection to isolate mutants that mislocalized a nuclear-targeted protein. We identified temperature-sensitive mutants that accumulated several different nuclear proteins in the cytoplasm when shifted to the semipermissi...
Topics
- Amino Acid Sequence
- Biological Transport
- Cell Nucleus
- Cloning, Molecular
- Genes, Fungal
- Microscopy, Electron
- Molecular Sequence Data
- Mutation
- Nuclear Envelope
- Nuclear Pore Complex Proteins
- Nuclear Proteins
- Nucleocytoplasmic Transport Proteins