Isolation of the gene HEM4 encoding uroporphyrinogen III synthase in Saccharomyces cerevisiae.

Abstract excerpt

We have isolated a genomic DNA fragment that complements the yeast temperature-sensitive cyt mutation, causing respiratory deficiency and accumulation of porphyrins (Sugimura et al., 1966). Partial DNA sequencing of the complementing region and search for similarity in the DNA and protein databases revealed that (1) the gene had been previously isolated by complementation of the mutation ts2326 (Langgut et al.,...

Topics

• Base Sequence
• DNA, Fungal
• Genes, Fungal
• Genetic Complementation Test
• Molecular Sequence Data
• Mutation
• Saccharomyces cerevisiae
• Uroporphyrinogen III Synthetase