A one-step coupled amplification and oligonucleotide ligation procedure for multiplex genetic typing.

doi:10.1101/gr.4.6.337

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Article

A one-step coupled amplification and oligonucleotide ligation procedure for multiplex genetic typing.

PCR methods and applications - 1 Jun 1995

Eggerding F A

Abstract excerpt

A new technique, coupled amplification and oligonucleotide ligation (CAL), has been developed that allows for simultaneous multiplex amplification and genotyping of DNA. CAL is a biphasic method that combines in one assay DNA amplification by PCR with DNA genotyping by the oligonucleotide ligatio...

Topics

Alleles
Base Sequence
Cystic Fibrosis
DNA
DNA Primers
Exons
Genome, Human
Genotype
Humans
Molecular Sequence Data
Mouth Mucosa
Mutation
Nucleic Acid Denaturation
Oligonucleotide Probes
Polymerase Chain Reaction
Sensitivity and Specificity
Spectrometry, Fluorescence
Temperature

Source

Open in PubMed

Identifiers

PMID: 7580927
DOI: 10.1101/gr.4.6.337

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7580927-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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