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A one-step coupled amplification and oligonucleotide ligation procedure for multiplex genetic typing.

PCR methods and applications - 1 Jun 1995

Eggerding F A

Abstract excerpt

A new technique, coupled amplification and oligonucleotide ligation (CAL), has been developed that allows for simultaneous multiplex amplification and genotyping of DNA. CAL is a biphasic method that combines in one assay DNA amplification by PCR with DNA genotyping by the oligonucleotide ligation assay (OLA). By virtue of a difference in the melting temperatures of PCR primer-target DNA and OLA probe-target DNA...

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PMID: 7580927
DOI: 10.1101/gr.4.6.337

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