High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.

doi:10.1093/nar/22.21.4527

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Article

High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation.

Nucleic acids research - 25 Oct 1994

Grossman P D, Bloch W, Brinson E, Chang C C, Eggerding F A, Fung S, Iovannisci D M, Woo S, Winn-Deen E S, Iovannisci D A

Abstract excerpt

We describe a non-isotopic, semi-automated method for large-scale multiplex analysis of nucleic acid sequences, using the cystic fibrosis transmembrane regulator (CFTR) gene as an example. Products of a multiplex oligonucleotide ligation assay (OLA) are resolved electrophoretically from one another and from unligated probes under denaturing conditions with fluorescence detection. One ligation probe for each OLA...

Topics

Autoanalysis
Base Sequence
Cell Line, Transformed
Cystic Fibrosis Transmembrane Conductance Regulator
DNA
Electrophoresis
Exons
Fluorescent Dyes
Herpesvirus 4, Human
Membrane Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Nucleic Acid Denaturation

Oligonucleotide Probes

Oligonucleotides

Polymerase Chain Reaction

Sequence Analysis, DNA

Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 7526344
DOI: 10.1093/nar/22.21.4527
PMCID: PMC308489

Genes

CFTR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7526344-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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