Improvements to bead-based oligonucleotide ligation SNP genotyping assays.

doi:10.2144/000112960

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Article

Improvements to bead-based oligonucleotide ligation SNP genotyping assays.

BioTechniques - 1 Nov 2008

Bruse Shannon, Moreau Michael, Azaro Marco, Zimmerman Ray, Brzustowicz Linda

Abstract excerpt

We describe a bead-based, multiplexed, oligonucleotide ligation assay (OLA) performed on the Luminex flow cytometer. Differences between this method and those previously reported include the use of far fewer beads and the use of a universal oligonucleotide for signal detection. These innovations serve to significantly reduce the cost of the assay, while maintaining robustness and accuracy. Comparisons are made...

Topics

Alleles
Biological Assay
Flow Cytometry
Fluorescent Dyes
Genetic Techniques
Genome, Human
Genotype
Humans
Microspheres
Oligonucleotides
Polymorphism, Single Nucleotide

Source

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Identifiers

PMID: 19007340
DOI: 10.2144/000112960
PMCID: PMC3987951

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