Article
HLA-Cw allele analysis by PCR-restriction fragment length polymorphism: study of known and additional alleles.
Proceedings of the National Academy of Sciences of the United States of America - 12 Sept 1995
Tatari Z, Fortier C, Bobrynina V, Loiseau P, Charron D, Raffoux C
Abstract excerpt
We describe a technique for HLA-Cw genotyping by digestion of PCR-amplified genes with restriction endonucleases. Locus-specific primers selectively amplified HLA-Cw sequences from exon 2 in a single PCR that avoided coamplification of other classical and nonclassical class I genes. Amplified DNAs were digested with selected enzymes. Sixty-three homozygous cell lines from International Histocompatibility Workshop...
Topics
- Alleles
- B-Lymphocytes
- Base Sequence
- Cell Line
- Exons
- Gene Frequency
- Genotype
- HLA-C Antigens
- Heterozygote
- Histocompatibility Testing
- Homozygote