Article
Comparison of typing results by serology and polymerase chain reaction with sequence-specific primers for HLA-Cw in 650 individuals.
Tissue antigens - 1 Oct 1997
Mytilineos J, Christ U, Lempert M, Opelz G
Abstract excerpt
HLA-Cw typing by standard serological techniques is associated with a high frequency of blanks, and reliable typing reagents for several of the Cw specificities are scarce. We evaluated the PCR-SSP technique for Cw typing in 370 kidney transplant patients and 280 healthy blood donors. Serological...
Topics
- Alleles
- Blood Donors
- DNA Primers
- DNA Probes, HLA
- Diagnostic Errors
- Evaluation Studies as Topic
- Gene Frequency
- Genes, MHC Class I
- Genotype
- HLA-C Antigens
- Histocompatibility Testing
- Humans
- Kidney Transplantation
- Phenotype
- Polymerase Chain Reaction
- Reagent Kits, Diagnostic
- Sensitivity and Specificity
- Serologic Tests