Article
The urease locus of Mycobacterium tuberculosis and its utilization for the demonstration of allelic exchange in Mycobacterium bovis bacillus Calmette-Guérin.
Proceedings of the National Academy of Sciences of the United States of America - 12 Sept 1995
Reyrat J M, Berthet F X, Gicquel B
Abstract excerpt
The ureABC genes of Mycobacterium tuberculosis were cloned. By using a set of degenerate primers corresponding to a conserved region of the urease enzyme (EC 3.5.1.5), a fragment of the expected size was amplified by PCR and was used to screen a M. tuberculosis cosmid library. Three open reading...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Chromosome Mapping
- Genome, Bacterial
- Genomic Library
- Genotype
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mycobacterium bovis
- Mycobacterium tuberculosis
- Polymerase Chain Reaction
- Recombination, Genetic