Article
Segregation of unreplicated chromosomes in Saccharomyces cerevisiae reveals a novel G1/M-phase checkpoint.
Molecular and cellular biology - 1 Oct 1995
Toyn J H, Johnson A L, Johnston L H
Abstract excerpt
Saccharomyces cerevisiae dbf4 and cdc7 cell cycle mutants block initiation of DNA synthesis (i.e., are iDS mutants) at 37 degrees C and arrest the cell cycle with a 1C DNA content. Surprisingly, certain dbf4 and cdc7 strains divide their chromatin at 37 degrees C. We found that the activation of the Cdc28 mitotic protein kinase and the Dbf2 kinase occurred with the correct relative timing with respect to each...
Topics
- CDC28 Protein Kinase, S cerevisiae
- Cell Cycle Proteins
- Chromosomes, Fungal
- DNA Replication
- Enzyme Activation
- Fungal Proteins
- G1 Phase
- Histidine Kinase
- Hydroxyurea
- Mitosis
- Models, Genetic