An inducible acid phosphatase from the yeast Pichia pastoris: characterization of the gene and its product.

doi:10.1016/0378-1119(95)00379-k

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Article

An inducible acid phosphatase from the yeast Pichia pastoris: characterization of the gene and its product.

Gene - 22 Sept 1995

Payne W E, Gannon P M, Kaiser C A

Abstract excerpt

To develop the budding yeast Pichia pastoris (Pp) as a model system for the study of protein secretion, we have characterized a secreted acid phosphatase (Pho1p) from this yeast. Pho1p can be induced 100-fold by starvation for phosphate. The enzyme was purified to homogeneity from a cell-wall ext...

Topics

Acid Phosphatase
Amino Acid Sequence
Base Sequence
DNA, Fungal
Enzyme Induction
Gene Library
Genes, Fungal
Genetic Complementation Test
Genotype
Kinetics
Molecular Sequence Data
Mutagenesis
Pichia
Plasmids
Restriction Mapping
Sequence Homology, Amino Acid

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