Article
Mutation of the metal-bridging proton-donor His63 residue in human Cu, Zn superoxide dismutase. Biochemical and biophysical analysis of the His63-->Cys mutant.
European journal of biochemistry - 15 Aug 1995
Banci L, Bertini I, Borsari M, Viezzoli M S, Hallewell R A
Abstract excerpt
The bridging His63 residue in human Cu, Zn superoxide dismutase, which binds both metals, has been replaced by a Cys residue. The mutant protein has been purified from Escherichia coli and appears to be a normal dimer. Spectroscopic techniques (electronic spectroscopies, EPR, nuclear magnetic relaxation dispersion) show that Cys63 binds the zinc ion, but not the copper ion, and that the latter is probably five...
Topics
- Copper
- Cysteine
- Enzyme Activation
- Histidine
- Humans
- Mutation
- Protons
- Superoxide Dismutase
- Zinc