Article
Human ferrochelatase: characterization of substrate-iron binding and proton-abstracting residues.
Biochemistry - 21 Aug 2001
Sellers V M, Wu C K, Dailey T A, Dailey H A
Abstract excerpt
The terminal step in heme biosynthesis, the insertion of ferrous iron into protoporphyrin IX to form protoheme, is catalyzed by the enzyme ferrochelatase (EC 4.99.1.1). A number of highly conserved residues identified from the crystal structure of human ferrochelatase as being in the active site were examined by site-directed mutagenesis. The mutants Y123F, Y165F, Y191H, and R164L each had an increased K(m) for...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.