Article
The bldA-encoded tRNA is poorly expressed in the bldI mutant of Streptomyces coelicolor A3(2).
Microbiology (Reading, England) - 1 Aug 1995
Leskiw Brenda K, Mah Richard
Abstract excerpt
The bldA gene, encoding a leucyl tRNA recognizing the UUA codon, is expressed at significantly lower levels in the bldI mutant, Streptomyces coelicolor J703, than in the parent S. coelicolor A3(2). Expression of a TTA-containing reporter gene was reduced in the bldI mutant, as was the mature, 87 nucleotide, form of the bldA-encoded tRNA. This reduced level of the tRNA was also seen when the bldA gene was...
Topics
- Bacterial Proteins
- Base Sequence
- Blotting, Northern
- Gene Dosage
- Gene Expression Regulation, Bacterial
- Genes, Reporter
- Molecular Sequence Data
- Mutation
- Plasmids
- Polymerase Chain Reaction
- Protein Biosynthesis