Article
Enzyme evolution in Rhodobacter sphaeroides: selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme.
Microbiology (Reading, England) - 1 Aug 1995
Schneider Karl-Heinz, Jakel Gregor, Hoffmann Ralf, Giffhorn Friedrich
Abstract excerpt
A gain of function mutant of Rhodobacter sphaeroides Si4, capable of growing on galactitol, was isolated from a chemostat culture. Continuous cultivation was performed for 54 d with a limiting concentration (1 mM) of the substrate D-glucitol and an excess (20 mM) of the non-metabolizable galactit...
Topics
- Alcohols
- Amino Acid Sequence
- Biological Evolution
- Chromatography
- Electrophoresis, Polyacrylamide Gel
- Galactitol
- Isoelectric Point
- Kinetics
- Malates
- Molecular Sequence Data
- Monosaccharides
- Mutation
- Rhodobacter sphaeroides
- Sequence Alignment
- Sugar Alcohol Dehydrogenases
- Sugar Alcohols
- Time Factors