Article
An evolutionary optimization of a rhodopsin-based phototrophic metabolism in Escherichia coli.
Microbial cell factories - 15 Jun 2017
Kim Hyun Aaron, Kim Hyun Ju, Park Jihoon, Choi Ah Reum, Heo Kyoo, Jeong Haeyoung, Jung Kwang-Hwan, Seok Yeong-Jae, Kim Pil, Lee Sang Jun
Abstract excerpt
BACKGROUND: The expression of the Gloeobacter rhodopsin (GR) in a chemotrophic Escherichia coli enables the light-driven phototrophic energy generation. Adaptive laboratory evolution has been used for acquiring desired phenotype of microbial cells and for the elucidation of basic mechanism of molecular evolution. To develop an optimized strain for the artificially acquired phototrophic metabolism, an ancestral E....
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