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Article

Characterization and properties of G4X mutants of Ralstonia eutropha PHA synthase for poly(3-hydroxybutyrate) biosynthesis in Escherichia coli.

Macromolecular bioscience - 15 Mar 2005

Normi Yahaya M, Hiraishi Tomohiro, Taguchi Seiichi, Abe Hideki, Sudesh Kumar, Najimudin Nazalan, Doi Yoshiharu

Abstract excerpt

Modification of the type I polyhydroxyalkanoate synthase of Ralstonia eutropha (PhaC(Re)) was performed through systematic in vitro evolution in order to obtain improved PhaC(Re) having an enhanced activity of poly(3-hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli. For the first time, a beneficial G4D N-terminal mutation important for the enhancement of both PHB content in dry cells and PhaC(Re)...

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PMID: 15768438
DOI: 10.1002/mabi.200400181

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