Article
A microdissection approach to detect molecular markers during progression of prostate cancer.
British journal of cancer - 1 Oct 1995
Berthon P, Dimitrov T, Stower M, Cussenot O, Maitland N J
Abstract excerpt
To investigate the underlying mechanisms of carcinogenesis, we have developed a technique to determine the frequency of genetic changes in prostatic carcinoma tissue. We have demonstrated that at a ratio of between 1:4 and 1:9 mutant-normal alleles, the signal from a mutant TP53 allele is not apparent after polymerase chain reaction (PCR) amplification and further direct sequencing or single-strand conformation...
Topics
- Base Sequence
- Dissection
- Genes, p53
- Humans
- Male
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Prostatic Neoplasms