Classification of mutations at the human hprt-locus in T-lymphocytes of bus maintenance workers by multiplex-PCR and reverse transcriptase-PCR analysis.

Abstract excerpt

Polymerase chain reaction (PCR)-based screening methods were used to classify mutations arising in vivo at the hypoxanthine guanine phosphoribosyl-transferase (hprt) locus in small samples of human T-lymphocyte clones (< 5 x 10(4) cells) from 29 bus maintenance workers exposed to diesel exhaust, and 14 control individuals. All subjects were healthy, non-smoking males. Among 462 T-cell mutants studied by...

Topics

• Base Sequence
• DNA Adducts
• Gene Deletion
• Humans
• Hypoxanthine Phosphoribosyltransferase
• Male
• Molecular Sequence Data
• Mutation
• Occupational Exposure
• Polymerase Chain Reaction
• T-Lymphocytes