Article
Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR.
Journal of clinical microbiology - 1 Jan 1995
Cebula T A, Payne W L, Feng P
Abstract excerpt
Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-II genes in a multiplex PCR assay. Analysis of 108 bacteria showed that all Escherichia coli serotype O15...
Topics
- Bacterial Toxins
- Base Sequence
- DNA Primers
- Escherichia coli
- Evaluation Studies as Topic
- Genes, Bacterial
- Genetic Markers
- Glucuronidase
- Molecular Sequence Data
- Mutation
- O Antigens
- Polymerase Chain Reaction
- Polysaccharides, Bacterial
- Serotyping
- Shiga Toxin 1
- Shiga Toxin 2