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Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157:H7/H-using SYBR Green I in a real-time multiplex PCR.

Molecular and cellular probes - 1 Feb 2006

Yoshitomi Ken J, Jinneman Karen C, Weagant Stephen D

Abstract excerpt

Enterohemorrhagic Escherichia coli (EHEC) is a major foodborne pathogen capable of causing diarrhea and vomiting, but more serious complications such as hemorrhagic colitis and hemolytic-uremic syndrome (HUS) can result. A real-time PCR method to detect the presence of Shiga toxin producing E. coli (STEC) and E. coli O157:H7 was investigated using SYBR Green I (SG). Primers were designed to target the Shiga toxin...

Topics

Benzothiazoles
Diamines
Escherichia coli O157
Fluorescent Dyes
Genotype
Humans
Mutation
Nucleic Acid Denaturation
Organic Chemicals
Polymerase Chain Reaction
Quinolines

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Shiga Toxin 1

Shiga Toxin 2

Source

Identifiers

PMID: 16271448
DOI: 10.1016/j.mcp.2005.09.002

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-16271448-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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