Article
Identification of single base-pair mutation on uidA gene of Escherichia coli O157:H7 by Peptide Nucleic Acids (PNA) mediated PCR clamping.
Bioscience, biotechnology, and biochemistry - 1 Feb 2004
Takiya Toshiyuki, Futo Satoshi, Tsuna Mika, Namimatsu Takanori, Sakano Tetsuya, Kawai Keiichi, Suzuki Tohru
Abstract excerpt
Peptide Nucleic Acids (PNA) is a new type of DNA analogue with a peptide backbone. We developed a rapid identification system of Escherichia. coli O157:H7 using PNA mediated PCR clamping. Firstly, we confirmed a single nucleotide alteration in the uidA gene (T93G), which is specific to E. coli O157: H7. We designed forward mutant DNA primer, wild type PNA, and a reverse DNA primer corresponding to the uidA...
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