Article
Detecting and genotyping Escherichia coli O157:H7 using multiplexed PCR and nucleic acid microarrays.
International journal of food microbiology - 20 Jul 2001
Call D R, Brockman F J, Chandler D P
Abstract excerpt
Rapid detection and characterization of food borne pathogens such as Escherichia coli O157:H7 is crucial for epidemiological investigations and food safety surveillance. As an alternative to conventional technologies, we examined the sensitivity and specificity of nucleic acid microarrays for detecting and genotyping E. coli O157:H7. The array was composed of oligonucleotide probes (25-30 mer) complementary to...
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