Article
Two distinct pathways of specific killing revealed by perforin mutant cytotoxic T lymphocytes.
Immunity - 1 Aug 1994
Kojima H, Shinohara N, Hanaoka S, Someya-Shirota Y, Takagaki Y, Ohno H, Saito T, Katayama T, Yagita H, Okumura K
Abstract excerpt
To study the contribution of putative perforin-independent mechanism in the antigen-specific target destruction by cytotoxic T lymphocytes CD8+ CTL lines were established from spleen cells of chimeric mice produced by injecting perforin (-/-) embryonic stem cells into blastocysts of RAG-2(-/-) mice. When tested on normal concanavalin A blasts, these perforin-deficient cytotoxic T lymphocyte lines were found to be...
Topics
- Animals
- Antigens, Surface
- Cell Death
- Cytotoxicity, Immunologic
- Humans
- Membrane Glycoproteins
- Mice
- Mice, Inbred DBA
- Mutation
- Perforin
- Pore Forming Cytotoxic Proteins