Article
Essential roles of perforin in antigen-specific cytotoxicity mediated by human CD4+ T lymphocytes: analysis using the combination of hereditary perforin-deficient effector cells and Fas-deficient target cells.
Journal of immunology (Baltimore, Md. : 1950) - 15 Feb 2003
Yanai Fumio, Ishii Eiichi, Kojima Kensuke, Hasegawa Atsuhiko, Azuma Taichi, Hirose Shinichi, Suga Naohiro, Mitsudome Akihisa, Zaitsu Masafumi, Ishida Yasushi, Shirakata Yuji, Sayama Koji, Hashimoto Koji, Yasukawa Masaki
Abstract excerpt
Although the cytotoxic mechanisms of murine CTLs have been investigated extensively using various mutant and knockout mice, those of human CTLs, especially CD4+ CTLs, are still obscure. To clarify the roles of perforin in Ag-specific cytotoxicity mediated by human CD4+ CTLs, alloantigen-specific and HSV-specific human CD4+ T lymphocyte bulk lines and clones were established from a patient with hereditary perforin...
Topics
- B-Lymphocytes
- CD8-Positive T-Lymphocytes
- Cell Line, Transformed
- Cells, Cultured
- Clone Cells
- Coculture Techniques
- Cytotoxicity Tests, Immunologic
- Cytotoxicity, Immunologic