Article
Human immunodeficiency virus reverse transcriptase substitutes for DNA polymerase I in Escherichia coli.
Proceedings of the National Academy of Sciences of the United States of America - 31 Jan 1995
Kim B, Loeb L A
Abstract excerpt
We present evidence that human immunodeficiency virus (HIV) reverse transcriptase (RT) can substitute for DNA polymerase I in bacteria. Expression of HIV RT enables an Escherichia coli mutant, polA12 recA718, containing a temperature-sensitive mutation in DNA polymerase I, to grow at a nonpermiss...
Topics
- DNA Polymerase I
- DNA Replication
- Drug Evaluation, Preclinical
- Escherichia coli
- Genetic Complementation Test
- HIV
- Humans
- Mutation
- Plasmids
- RNA-Directed DNA Polymerase
- Temperature
- Zidovudine