Article
Catalytic activity and transformation potential of v-Src require arginine 385 in the substrate binding pocket.
Oncogene - 5 Jan 1995
Senften M, Schenker G, Sowadski J M, Ballmer-Hofer K
Abstract excerpt
Tyrosine kinase are important mediators of signal transduction in eukaryotic cells. In order to better understand the mechanism of catalysis we studied a set of mutants of the prototype tyrosine kinase, the c-Src protein, a homologue of the Rous Sarcoma virus oncogene. Based on an X-ray structure of cAMP-dependent protein kinase (cAPK) we mutated an arginine residue conserved in subdomain VI of all known kinases...
Topics
- 3T3 Cells
- Animals
- Arginine
- Avian Sarcoma Viruses
- Base Sequence
- Binding Sites
- Catalysis
- Cell Transformation, Neoplastic
- Cell Transformation, Viral
- Cells, Cultured
- Mice