Article
Two identical noninteracting sites in an ion channel revealed by proton transfer.
Science (New York, N.Y.) - 23 Sept 1994
Root M J, MacKinnon R
Abstract excerpt
The functional consequences of single proton transfers occurring in the pore of a cyclic nucleotide-gated channel were observed with patch recording techniques. These results led to three conclusions about the chemical nature of ion binding sites in the conduction pathway: The channel contains two identical titratable sites, even though there are more than two (probably four) identical subunits; the sites are...
Topics
- Amino Acid Sequence
- Animals
- Binding Sites
- Calcium Channels
- Catfishes
- Electric Conductivity
- Hydrogen-Ion Concentration
- Ion Channel Gating
- Ion Channels
- Kinetics
- Molecular Sequence Data