Detection of calcium binding proteins on polyacrylamide gels using time-resolved lanthanide luminescence photography.

doi:10.1006/abio.1994.1065

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Article

Detection of calcium binding proteins on polyacrylamide gels using time-resolved lanthanide luminescence photography.

Analytical biochemistry - 1 Feb 1994

Hill I E, Hogue C W, Clark I D, MacManus J P, Szabo A G

Abstract excerpt

Methods were developed for using the luminescent lanthanides Tb3+ and Eu3+ for the specific staining of calcium-binding proteins, as well as the nonspecific staining of proteins, on polyacrylamide gels. These methods involve equilibration of the gel after electrophoresis in solutions containing t...

Topics

Acrylic Resins
Calcium-Binding Proteins
Chelating Agents
Europium
Luminescent Measurements
Metals, Rare Earth
Mutation
Photography
Sensitivity and Specificity
Sodium Dodecyl Sulfate
Staining and Labeling
Terbium

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