Enzyme-dependent pausing during in vitro replication of O4-methylthymine in a defined oligonucleotide sequence.

doi:10.1016/0027-5107(94)90277-1

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Article

Enzyme-dependent pausing during in vitro replication of O4-methylthymine in a defined oligonucleotide sequence.

Mutation research - 1 May 1994

Menichini P, Mroczkowska M M, Singer B

Abstract excerpt

We had previously reported that an oligonucleotide containing a site-specifically incorporated O4-methylthymine (m4T) was replicated under kinetic conditions by the Klenow fragment of E. coli DNA polymerase I (Kf) (Dosanjh et al., 1993). Using other polymerases for complete replication, but with...

Topics

Base Sequence
DNA Replication
DNA-Directed DNA Polymerase
Kinetics
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Thymine

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