Article
Lead-catalyzed cleavage of ribonuclease P RNA as a probe for integrity of tertiary structure.
Nucleic acids research - 25 Dec 1993
Zito K, Hüttenhofer A, Pace N R
Abstract excerpt
Pb(2+)-catalyzed cleavage of RNA has been shown previously to be a useful probe for tertiary structure. In the present study, Pb2+ cleavage patterns were identified for ribonuclease P RNAs from three phylogenetically disparate organisms, Escherichia coli, Chromatium vinosum, Bacillus subtilis, and for E. coli RNase P RNAs that had been altered by deletions. Each of the native RNAs undergoes cleavage at several...
Topics
- Base Sequence
- Catalysis
- DNA, Bacterial
- Endoribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Lead
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Phylogeny