Identification of endoribonuclease specific cleavage positions reveals novel targets of RNase III inStreptococcus pyogenes

Abstract excerpt

A better understanding of transcriptional and post-transcriptional regulation of gene expression in bacteria relies on studying their transcriptome. RNA sequencing methods are used not only to assess RNA abundance but also the exact boundaries of primary and processed transcripts. Here, we developed a method, called identification of specific cleavage position (ISCP), which enables the identification of direct...

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