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Article

Evidence of surface antigen detachment during incubation of cells with immunomagnetic beads.

Journal of immunological methods - 3 Dec 1993

Rubbi C P, Patel D, Rickwood D

Abstract excerpt

We have studied the attachment of immunomagnetic beads to different cells, with particular interest in cells that did not, as expected, appear to bind antibody-coated beads. Through the use of immunofluorescence and laser scanning confocal microscopy it was possible to demonstrate that beads can detach significant amounts of antigen from the surface of cells. This results in the appearance of antigen-depleted yet...

Topics

Antigens, CD
Antigens, CD19
Antigens, Differentiation, B-Lymphocyte
Antigens, Surface
Cell Separation
Cell Survival
Fluorescein
Fluoresceins
Humans
Immunomagnetic Separation
Microscopy

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 7507146
DOI: 10.1016/0022-1759(93)90364-d

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7507146-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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