Article
Analysis of in vitro lymphocyte adhesion and transendothelial migration by fluorescent-beads-based flow cytometric cell counting.
Cytometry - 1 May 1998
Molema G, Mesander G, Kroesen B J, Helfrich W, Meijer D K, de Leij L F
Abstract excerpt
In this report, we describe a new and simple method for flow cytometric quantitation of lymphocyte numbers in lymphocyte-endothelial adhesion/ transendothelial migration assays. The method exploits fluorescent flow cytometer alignment beads as a counting reference. Known amounts of beads are adde...
Topics
- Antibodies, Monoclonal
- Antigens, Neoplasm
- CD3 Complex
- Cell Adhesion
- Cell Adhesion Molecules
- Cell Count
- Cell Movement
- Cell Separation
- Cells, Cultured
- Endothelium, Vascular
- Epithelial Cell Adhesion Molecule
- Flow Cytometry
- Humans
- Immunoglobulin Fab Fragments
- Lymphocyte Activation
- Lymphocytes
- Microspheres
- Phenotype