Article
Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function.
Journal of visualized experiments : JoVE - 16 Feb 2017
Smith Mia J, Packard Thomas A, O'Neill Shannon K, Hinman Rochelle M, Rihanek Marynette, Gottlieb Peter A, Cambier John C
Abstract excerpt
B cells reactive with a specific antigen usually occur at a frequency of <0.05% of lymphocytes. For decades researchers have sought methods to isolate and enrich these rare cells for studies of their phenotype and biology. Approaches are inevitably based on the principle that B cells recognize native antigen by virtue of cell surface receptors that are representative in specificity of antibodies that will...
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