Detection of a novel point mutation causing haemophilia A by PCR/direct sequencing of ectopically-transcribed factor VIII mRNA.

doi:10.1007/BF00193593

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Article

Detection of a novel point mutation causing haemophilia A by PCR/direct sequencing of ectopically-transcribed factor VIII mRNA.

Human genetics - 1 Oct 1990

Berg L P, Wieland K, Millar D S, Schlösser M, Wagner M, Kakkar V V, Reiss J, Cooper D N

Abstract excerpt

Specifically-primed reverse transcripts of lymphocyte-derived factor VIII (FVIII) mRNA were successfully amplified by means of the polymerase chain reaction (PCR) thus further extending the phenomenon of ectopic ("illegitimate") transcription of tissue-specific genes. The diagnostic potential of...

Topics

Base Sequence
Factor VIII
Hemophilia A
Humans
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
RNA, Messenger
Transcription, Genetic

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