Article
Splicing variability in HIV type 1 revealed by quantitative RNA polymerase chain reaction.
AIDS research and human retroviruses - 1 Nov 1994
Neumann M, Harrison J, Saltarelli M, Hadziyannis E, Erfle V, Felber B K, Pavlakis G N
Abstract excerpt
A quantitative RNA-polymerase chain reaction (PCR) method able to detect the majority of mRNAs produced by human immunodeficiency virus type 1 (HIV-1) was developed and used to study expression of different HIV-1 clones in human cells. Amplified mRNAs were compared to known cDNA standards. This comparison permitted the optimization of PCR conditions and eliminated the generation of artifactual PCR bands. The use...
Topics
- Base Sequence
- Cell Line
- DNA Primers
- DNA, Complementary
- DNA, Viral
- Genetic Variation
- HIV Infections
- HIV-1
- HeLa Cells
- Humans
- In Vitro Techniques