Article
Use of spontaneously mutated human DNA as competitive internal standard for nucleic acid quantification by reverse transcription-polymerase chain reaction (RT-PCR).
Archivum immunologiae et therapiae experimentalis - 1 Jan 1995
Rudnicka L, Diaz A, Varga J, Christiano A, Uitto J, Jimenez S A
Abstract excerpt
Quantification of gene expression is of increasing interest in many medical sciences. Methods based on reverse transcription-polymerase chain reactions (RT-PCRs) are timesaving and require only very small amounts of RNA. A limiting factor, however, is the significant fluctuation in the efficacy of reverse transcription as well as in the polymerase chain reactions. Various external and internal standards have been...
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