Article
Use of spontaneously mutated human DNA as competitive internal standard for nucleic acid quantification by reverse transcription-polymerase chain reaction (RT-PCR).
Archivum immunologiae et therapiae experimentalis - 1 Jan 1995
Rudnicka L, Diaz A, Varga J, Christiano A, Uitto J, Jimenez S A
Abstract excerpt
Quantification of gene expression is of increasing interest in many medical sciences. Methods based on reverse transcription-polymerase chain reactions (RT-PCRs) are timesaving and require only very small amounts of RNA. A limiting factor, however, is the significant fluctuation in the efficacy o...
Topics
- Base Sequence
- Collagen
- DNA
- Fibroblasts
- Humans
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- RNA, Messenger
- Reference Standards
- Reference Values
- Scleroderma, Systemic
- Transcription, Genetic
- Transforming Growth Factor beta