Article
Low frequency of mutations in the WT1 coding region in Wilms' tumor.
Genes, chromosomes & cancer - 1 Oct 1993
Brown K W, Wilmore H P, Watson J E, Mott M G, Berry P J, Maitland N J
Abstract excerpt
A series of twenty unselected Wilms' tumors were analysed for alterations in the WT1 tumor suppressor gene. The entire coding region of WT1 was amplified by RNA-PCR, and then screened for mutations by single-strand conformational polymorphism analysis (SSCP). This method was shown to be capable o...
Topics
- Genes, Wilms Tumor
- Humans
- Kidney Neoplasms
- Mutation
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- RNA
- Transcription, Genetic
- Wilms Tumor