Article
Isolation of temperature-sensitive mutants for mRNA capping enzyme in Saccharomyces cerevisiae.
Molecular & general genetics : MGG - 15 Nov 1995
Yamagishi M, Mizumoto K, Ishihama A
Abstract excerpt
The guanylyltransferase activity of mRNA capping enzyme catalyzes the transfer of GMP from GTP to the 5' terminus of mRNA. In Saccharomyces cerevisiae, the activity is carried on the alpha subunit of capping enzyme, the product of the CEG1 gene. We have isolated 10 recessive, temperature-sensitiv...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosomes, Fungal
- DNA Primers
- Genes, Fungal
- Genes, Recessive
- Genotype
- Kinetics
- Molecular Sequence Data
- Mutation
- Nucleotidyltransferases
- Open Reading Frames
- Polymerase Chain Reaction
- Restriction Mapping
- Saccharomyces cerevisiae
- Schizosaccharomyces
- Sequence Homology, Amino Acid
- Temperature