Article
An efficient method to isolate yeast genes causing overexpression-mediated growth arrest.
Yeast (Chichester, England) - 1 Jan 1995
Espinet C, de la Torre M A, Aldea M, Herrero E
Abstract excerpt
In order to characterize new yeast genes regulating cell proliferation, a number of overexpression-sensitive clones have been isolated from a Saccharomyces cerevisiae cDNA library in a multicopy vector under the control of the GAL1 promoter, on the basis of growth arrest phenotype under galactose...
Topics
- Cell Division
- DNA-Binding Proteins
- Flow Cytometry
- Galactose
- Gene Expression Regulation, Fungal
- Genes, Dominant
- Genetic Vectors
- Genomic Library
- Glucose
- Growth Inhibitors
- Minichromosome Maintenance 1 Protein
- Molecular Sequence Data
- Phenotype
- Promoter Regions, Genetic
- Saccharomyces cerevisiae
- Transcription Factors
- Transformation, Genetic