Article
Cold-sensitive mutants G680V and G691C of Dictyostelium myosin II confer dramatically different biochemical defects.
The Journal of biological chemistry - 31 Oct 1997
Patterson B, Ruppel K M, Wu Y, Spudich J A
Abstract excerpt
Cold-sensitive myosin mutants represent powerful tools for dissecting discrete deficiencies in myosin function. Biochemical characterization of two such mutants, G680V and G691C, has allowed us to identify separate facets of myosin motor function perturbed by each alteration. Compared with wild t...
Topics
- Actins
- Amino Acid Sequence
- Animals
- Calcium-Transporting ATPases
- Cold Temperature
- Dictyostelium
- Glycine
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Myosins
- Protein Binding